Review



hsp60  (Cell Signaling Technology Inc)


Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 97
      Buy from Supplier

    Structured Review

    Cell Signaling Technology Inc hsp60
    Hsp60, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 364 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hsp60/product/Cell Signaling Technology Inc
    Average 97 stars, based on 364 article reviews
    hsp60 - by Bioz Stars, 2026-02
    97/100 stars

    Images



    Similar Products

    rabbit anti hspd1  (Proteintech)
    96
    Proteintech rabbit anti hspd1
    Rabbit Anti Hspd1, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti hspd1/product/Proteintech
    Average 96 stars, based on 1 article reviews
    rabbit anti hspd1 - by Bioz Stars, 2026-02
    96/100 stars
    		  Buy from Supplier
    rabbit polyclonal hsp60 antibody  (Bio-Rad)
    96
    Bio-Rad rabbit polyclonal hsp60 antibody
    (A) HSF1-/- cells exhibit reduced expression of heat shock inducible Hsps. WT and HSF1-/- MEF cells were exposed to heat shock at 45 °C for 15 min and allowed to recover at 37 °C for the indicated times. Hsp90, Grp78, Hsc/Hsp70, <t>Hsp60,</t> Hsp40, and Hsp27 were detected by Western blot analysis with their specific antibodies. (B, C) Hsp70 participates in UPS-dependent Arc/Arg3.1 degradation. HSF1-/- MEF cells overexpressing Flag-Hsp70 were exposed to heat shock at 45 °C for 15 min and allowed to recover at 37 °C for the indicated times. Flag-Hsp70 and Arc/Arg3.1 were detected by Western blot analysis using their specific antibodies. Representative Western results were selected from triplicated experiments. Quantified results of triplicated Western images are shown in (C). (D) Hsp70 interacts with Arc/Arg3.1. HEK293T cells were transfected with GFP-Hsp70 and Flag empty vector or Flag-Arc, and the cell lysates were immunoprecipitated using an anti-Flag antibody. The immune complex was analyzed by Western analysis using anti-GFP and anti-Flag antibodies. (E) Arc/Arg3.1 primarly binds to Hsc/Hsp70. Following transfection of HEK293T cells with either Flag empty vector or Flag-Arc, the whole cell lysates were immunoprecipitated using an anti-Flag antibody. Western analysis using anti-Hsc/Hsp70, anti-Hsp40, and anti-Hsp27 antibodies was used to examine the immunological complex. * was an antibody’s light chain. (F, G) CHIP induces Arc/Arg3.1 degradation by interacting with Hsp70. HEK293T cells overexpressing Flag-Arc, GFP-Hsp70, and His-ubiquitin were co-transfected with HA-CHIP WT or mutants. Cells were analyzed by Western blot with anti-Flag, anti-HA, and anti-GFP antibodies. Representative Western results were selected from triplicated experiments. Quantified results of triplicated Western images are shown in (G). Data are presented as the mean ± S.D. of triplicated experiments (t-test; # < 0.01, **P < 0.001, *** < 0.0001). (H) Arc/Arg3.1 proteins are stabilized by CHIP knockdown. After being depleted of either control or CHIP, MEF cells were exposed to heat shock at 45°C for 15 min and then allowed to recover at 37°C for 9 h.
    Rabbit Polyclonal Hsp60 Antibody, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal hsp60 antibody/product/Bio-Rad
    Average 96 stars, based on 1 article reviews
    rabbit polyclonal hsp60 antibody - by Bioz Stars, 2026-02
    96/100 stars
    		  Buy from Supplier
    rabbit anti hsp60 igg  (Proteintech)
    96
    Proteintech rabbit anti hsp60 igg
    (A) HSF1-/- cells exhibit reduced expression of heat shock inducible Hsps. WT and HSF1-/- MEF cells were exposed to heat shock at 45 °C for 15 min and allowed to recover at 37 °C for the indicated times. Hsp90, Grp78, Hsc/Hsp70, <t>Hsp60,</t> Hsp40, and Hsp27 were detected by Western blot analysis with their specific antibodies. (B, C) Hsp70 participates in UPS-dependent Arc/Arg3.1 degradation. HSF1-/- MEF cells overexpressing Flag-Hsp70 were exposed to heat shock at 45 °C for 15 min and allowed to recover at 37 °C for the indicated times. Flag-Hsp70 and Arc/Arg3.1 were detected by Western blot analysis using their specific antibodies. Representative Western results were selected from triplicated experiments. Quantified results of triplicated Western images are shown in (C). (D) Hsp70 interacts with Arc/Arg3.1. HEK293T cells were transfected with GFP-Hsp70 and Flag empty vector or Flag-Arc, and the cell lysates were immunoprecipitated using an anti-Flag antibody. The immune complex was analyzed by Western analysis using anti-GFP and anti-Flag antibodies. (E) Arc/Arg3.1 primarly binds to Hsc/Hsp70. Following transfection of HEK293T cells with either Flag empty vector or Flag-Arc, the whole cell lysates were immunoprecipitated using an anti-Flag antibody. Western analysis using anti-Hsc/Hsp70, anti-Hsp40, and anti-Hsp27 antibodies was used to examine the immunological complex. * was an antibody’s light chain. (F, G) CHIP induces Arc/Arg3.1 degradation by interacting with Hsp70. HEK293T cells overexpressing Flag-Arc, GFP-Hsp70, and His-ubiquitin were co-transfected with HA-CHIP WT or mutants. Cells were analyzed by Western blot with anti-Flag, anti-HA, and anti-GFP antibodies. Representative Western results were selected from triplicated experiments. Quantified results of triplicated Western images are shown in (G). Data are presented as the mean ± S.D. of triplicated experiments (t-test; # < 0.01, **P < 0.001, *** < 0.0001). (H) Arc/Arg3.1 proteins are stabilized by CHIP knockdown. After being depleted of either control or CHIP, MEF cells were exposed to heat shock at 45°C for 15 min and then allowed to recover at 37°C for 9 h.
    Rabbit Anti Hsp60 Igg, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti hsp60 igg/product/Proteintech
    Average 96 stars, based on 1 article reviews
    rabbit anti hsp60 igg - by Bioz Stars, 2026-02
    96/100 stars
    		  Buy from Supplier
    polyclonal rabbit anti-hsp60 antibody novus nbp2-12734  (Novus Biologicals)
    90
    Novus Biologicals polyclonal rabbit anti-hsp60 antibody novus nbp2-12734
    (A) HSF1-/- cells exhibit reduced expression of heat shock inducible Hsps. WT and HSF1-/- MEF cells were exposed to heat shock at 45 °C for 15 min and allowed to recover at 37 °C for the indicated times. Hsp90, Grp78, Hsc/Hsp70, <t>Hsp60,</t> Hsp40, and Hsp27 were detected by Western blot analysis with their specific antibodies. (B, C) Hsp70 participates in UPS-dependent Arc/Arg3.1 degradation. HSF1-/- MEF cells overexpressing Flag-Hsp70 were exposed to heat shock at 45 °C for 15 min and allowed to recover at 37 °C for the indicated times. Flag-Hsp70 and Arc/Arg3.1 were detected by Western blot analysis using their specific antibodies. Representative Western results were selected from triplicated experiments. Quantified results of triplicated Western images are shown in (C). (D) Hsp70 interacts with Arc/Arg3.1. HEK293T cells were transfected with GFP-Hsp70 and Flag empty vector or Flag-Arc, and the cell lysates were immunoprecipitated using an anti-Flag antibody. The immune complex was analyzed by Western analysis using anti-GFP and anti-Flag antibodies. (E) Arc/Arg3.1 primarly binds to Hsc/Hsp70. Following transfection of HEK293T cells with either Flag empty vector or Flag-Arc, the whole cell lysates were immunoprecipitated using an anti-Flag antibody. Western analysis using anti-Hsc/Hsp70, anti-Hsp40, and anti-Hsp27 antibodies was used to examine the immunological complex. * was an antibody’s light chain. (F, G) CHIP induces Arc/Arg3.1 degradation by interacting with Hsp70. HEK293T cells overexpressing Flag-Arc, GFP-Hsp70, and His-ubiquitin were co-transfected with HA-CHIP WT or mutants. Cells were analyzed by Western blot with anti-Flag, anti-HA, and anti-GFP antibodies. Representative Western results were selected from triplicated experiments. Quantified results of triplicated Western images are shown in (G). Data are presented as the mean ± S.D. of triplicated experiments (t-test; # < 0.01, **P < 0.001, *** < 0.0001). (H) Arc/Arg3.1 proteins are stabilized by CHIP knockdown. After being depleted of either control or CHIP, MEF cells were exposed to heat shock at 45°C for 15 min and then allowed to recover at 37°C for 9 h.
    Polyclonal Rabbit Anti Hsp60 Antibody Novus Nbp2 12734, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal rabbit anti-hsp60 antibody novus nbp2-12734/product/Novus Biologicals
    Average 90 stars, based on 1 article reviews
    polyclonal rabbit anti-hsp60 antibody novus nbp2-12734 - by Bioz Stars, 2026-02
    90/100 stars
    		  Buy from Supplier
    hsp60  (Cell Signaling Technology Inc)
    97
    Cell Signaling Technology Inc hsp60
    (A) HSF1-/- cells exhibit reduced expression of heat shock inducible Hsps. WT and HSF1-/- MEF cells were exposed to heat shock at 45 °C for 15 min and allowed to recover at 37 °C for the indicated times. Hsp90, Grp78, Hsc/Hsp70, <t>Hsp60,</t> Hsp40, and Hsp27 were detected by Western blot analysis with their specific antibodies. (B, C) Hsp70 participates in UPS-dependent Arc/Arg3.1 degradation. HSF1-/- MEF cells overexpressing Flag-Hsp70 were exposed to heat shock at 45 °C for 15 min and allowed to recover at 37 °C for the indicated times. Flag-Hsp70 and Arc/Arg3.1 were detected by Western blot analysis using their specific antibodies. Representative Western results were selected from triplicated experiments. Quantified results of triplicated Western images are shown in (C). (D) Hsp70 interacts with Arc/Arg3.1. HEK293T cells were transfected with GFP-Hsp70 and Flag empty vector or Flag-Arc, and the cell lysates were immunoprecipitated using an anti-Flag antibody. The immune complex was analyzed by Western analysis using anti-GFP and anti-Flag antibodies. (E) Arc/Arg3.1 primarly binds to Hsc/Hsp70. Following transfection of HEK293T cells with either Flag empty vector or Flag-Arc, the whole cell lysates were immunoprecipitated using an anti-Flag antibody. Western analysis using anti-Hsc/Hsp70, anti-Hsp40, and anti-Hsp27 antibodies was used to examine the immunological complex. * was an antibody’s light chain. (F, G) CHIP induces Arc/Arg3.1 degradation by interacting with Hsp70. HEK293T cells overexpressing Flag-Arc, GFP-Hsp70, and His-ubiquitin were co-transfected with HA-CHIP WT or mutants. Cells were analyzed by Western blot with anti-Flag, anti-HA, and anti-GFP antibodies. Representative Western results were selected from triplicated experiments. Quantified results of triplicated Western images are shown in (G). Data are presented as the mean ± S.D. of triplicated experiments (t-test; # < 0.01, **P < 0.001, *** < 0.0001). (H) Arc/Arg3.1 proteins are stabilized by CHIP knockdown. After being depleted of either control or CHIP, MEF cells were exposed to heat shock at 45°C for 15 min and then allowed to recover at 37°C for 9 h.
    Hsp60, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hsp60/product/Cell Signaling Technology Inc
    Average 97 stars, based on 1 article reviews
    hsp60 - by Bioz Stars, 2026-02
    97/100 stars
    		  Buy from Supplier
    rabbit anti hsp60 antibody  (Proteintech)
    96
    Proteintech rabbit anti hsp60 antibody
    (A) HSF1-/- cells exhibit reduced expression of heat shock inducible Hsps. WT and HSF1-/- MEF cells were exposed to heat shock at 45 °C for 15 min and allowed to recover at 37 °C for the indicated times. Hsp90, Grp78, Hsc/Hsp70, <t>Hsp60,</t> Hsp40, and Hsp27 were detected by Western blot analysis with their specific antibodies. (B, C) Hsp70 participates in UPS-dependent Arc/Arg3.1 degradation. HSF1-/- MEF cells overexpressing Flag-Hsp70 were exposed to heat shock at 45 °C for 15 min and allowed to recover at 37 °C for the indicated times. Flag-Hsp70 and Arc/Arg3.1 were detected by Western blot analysis using their specific antibodies. Representative Western results were selected from triplicated experiments. Quantified results of triplicated Western images are shown in (C). (D) Hsp70 interacts with Arc/Arg3.1. HEK293T cells were transfected with GFP-Hsp70 and Flag empty vector or Flag-Arc, and the cell lysates were immunoprecipitated using an anti-Flag antibody. The immune complex was analyzed by Western analysis using anti-GFP and anti-Flag antibodies. (E) Arc/Arg3.1 primarly binds to Hsc/Hsp70. Following transfection of HEK293T cells with either Flag empty vector or Flag-Arc, the whole cell lysates were immunoprecipitated using an anti-Flag antibody. Western analysis using anti-Hsc/Hsp70, anti-Hsp40, and anti-Hsp27 antibodies was used to examine the immunological complex. * was an antibody’s light chain. (F, G) CHIP induces Arc/Arg3.1 degradation by interacting with Hsp70. HEK293T cells overexpressing Flag-Arc, GFP-Hsp70, and His-ubiquitin were co-transfected with HA-CHIP WT or mutants. Cells were analyzed by Western blot with anti-Flag, anti-HA, and anti-GFP antibodies. Representative Western results were selected from triplicated experiments. Quantified results of triplicated Western images are shown in (G). Data are presented as the mean ± S.D. of triplicated experiments (t-test; # < 0.01, **P < 0.001, *** < 0.0001). (H) Arc/Arg3.1 proteins are stabilized by CHIP knockdown. After being depleted of either control or CHIP, MEF cells were exposed to heat shock at 45°C for 15 min and then allowed to recover at 37°C for 9 h.
    Rabbit Anti Hsp60 Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti hsp60 antibody/product/Proteintech
    Average 96 stars, based on 1 article reviews
    rabbit anti hsp60 antibody - by Bioz Stars, 2026-02
    96/100 stars
    		  Buy from Supplier
    rabbit anti-hsp60  (Cell Signaling Technology Inc)
    90
    Cell Signaling Technology Inc rabbit anti-hsp60
    (A) HSF1-/- cells exhibit reduced expression of heat shock inducible Hsps. WT and HSF1-/- MEF cells were exposed to heat shock at 45 °C for 15 min and allowed to recover at 37 °C for the indicated times. Hsp90, Grp78, Hsc/Hsp70, <t>Hsp60,</t> Hsp40, and Hsp27 were detected by Western blot analysis with their specific antibodies. (B, C) Hsp70 participates in UPS-dependent Arc/Arg3.1 degradation. HSF1-/- MEF cells overexpressing Flag-Hsp70 were exposed to heat shock at 45 °C for 15 min and allowed to recover at 37 °C for the indicated times. Flag-Hsp70 and Arc/Arg3.1 were detected by Western blot analysis using their specific antibodies. Representative Western results were selected from triplicated experiments. Quantified results of triplicated Western images are shown in (C). (D) Hsp70 interacts with Arc/Arg3.1. HEK293T cells were transfected with GFP-Hsp70 and Flag empty vector or Flag-Arc, and the cell lysates were immunoprecipitated using an anti-Flag antibody. The immune complex was analyzed by Western analysis using anti-GFP and anti-Flag antibodies. (E) Arc/Arg3.1 primarly binds to Hsc/Hsp70. Following transfection of HEK293T cells with either Flag empty vector or Flag-Arc, the whole cell lysates were immunoprecipitated using an anti-Flag antibody. Western analysis using anti-Hsc/Hsp70, anti-Hsp40, and anti-Hsp27 antibodies was used to examine the immunological complex. * was an antibody’s light chain. (F, G) CHIP induces Arc/Arg3.1 degradation by interacting with Hsp70. HEK293T cells overexpressing Flag-Arc, GFP-Hsp70, and His-ubiquitin were co-transfected with HA-CHIP WT or mutants. Cells were analyzed by Western blot with anti-Flag, anti-HA, and anti-GFP antibodies. Representative Western results were selected from triplicated experiments. Quantified results of triplicated Western images are shown in (G). Data are presented as the mean ± S.D. of triplicated experiments (t-test; # < 0.01, **P < 0.001, *** < 0.0001). (H) Arc/Arg3.1 proteins are stabilized by CHIP knockdown. After being depleted of either control or CHIP, MEF cells were exposed to heat shock at 45°C for 15 min and then allowed to recover at 37°C for 9 h.
    Rabbit Anti Hsp60, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti-hsp60/product/Cell Signaling Technology Inc
    Average 90 stars, based on 1 article reviews
    rabbit anti-hsp60 - by Bioz Stars, 2026-02
    90/100 stars
    		  Buy from Supplier
    anti vdac  (Cell Signaling Technology Inc)
    94
    Cell Signaling Technology Inc anti vdac
    (A) HSF1-/- cells exhibit reduced expression of heat shock inducible Hsps. WT and HSF1-/- MEF cells were exposed to heat shock at 45 °C for 15 min and allowed to recover at 37 °C for the indicated times. Hsp90, Grp78, Hsc/Hsp70, <t>Hsp60,</t> Hsp40, and Hsp27 were detected by Western blot analysis with their specific antibodies. (B, C) Hsp70 participates in UPS-dependent Arc/Arg3.1 degradation. HSF1-/- MEF cells overexpressing Flag-Hsp70 were exposed to heat shock at 45 °C for 15 min and allowed to recover at 37 °C for the indicated times. Flag-Hsp70 and Arc/Arg3.1 were detected by Western blot analysis using their specific antibodies. Representative Western results were selected from triplicated experiments. Quantified results of triplicated Western images are shown in (C). (D) Hsp70 interacts with Arc/Arg3.1. HEK293T cells were transfected with GFP-Hsp70 and Flag empty vector or Flag-Arc, and the cell lysates were immunoprecipitated using an anti-Flag antibody. The immune complex was analyzed by Western analysis using anti-GFP and anti-Flag antibodies. (E) Arc/Arg3.1 primarly binds to Hsc/Hsp70. Following transfection of HEK293T cells with either Flag empty vector or Flag-Arc, the whole cell lysates were immunoprecipitated using an anti-Flag antibody. Western analysis using anti-Hsc/Hsp70, anti-Hsp40, and anti-Hsp27 antibodies was used to examine the immunological complex. * was an antibody’s light chain. (F, G) CHIP induces Arc/Arg3.1 degradation by interacting with Hsp70. HEK293T cells overexpressing Flag-Arc, GFP-Hsp70, and His-ubiquitin were co-transfected with HA-CHIP WT or mutants. Cells were analyzed by Western blot with anti-Flag, anti-HA, and anti-GFP antibodies. Representative Western results were selected from triplicated experiments. Quantified results of triplicated Western images are shown in (G). Data are presented as the mean ± S.D. of triplicated experiments (t-test; # < 0.01, **P < 0.001, *** < 0.0001). (H) Arc/Arg3.1 proteins are stabilized by CHIP knockdown. After being depleted of either control or CHIP, MEF cells were exposed to heat shock at 45°C for 15 min and then allowed to recover at 37°C for 9 h.
    Anti Vdac, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti vdac/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    anti vdac - by Bioz Stars, 2026-02
    94/100 stars
    		  Buy from Supplier
    anti hsp60  (Cell Signaling Technology Inc)
    97
    Cell Signaling Technology Inc anti hsp60
    (A) HSF1-/- cells exhibit reduced expression of heat shock inducible Hsps. WT and HSF1-/- MEF cells were exposed to heat shock at 45 °C for 15 min and allowed to recover at 37 °C for the indicated times. Hsp90, Grp78, Hsc/Hsp70, <t>Hsp60,</t> Hsp40, and Hsp27 were detected by Western blot analysis with their specific antibodies. (B, C) Hsp70 participates in UPS-dependent Arc/Arg3.1 degradation. HSF1-/- MEF cells overexpressing Flag-Hsp70 were exposed to heat shock at 45 °C for 15 min and allowed to recover at 37 °C for the indicated times. Flag-Hsp70 and Arc/Arg3.1 were detected by Western blot analysis using their specific antibodies. Representative Western results were selected from triplicated experiments. Quantified results of triplicated Western images are shown in (C). (D) Hsp70 interacts with Arc/Arg3.1. HEK293T cells were transfected with GFP-Hsp70 and Flag empty vector or Flag-Arc, and the cell lysates were immunoprecipitated using an anti-Flag antibody. The immune complex was analyzed by Western analysis using anti-GFP and anti-Flag antibodies. (E) Arc/Arg3.1 primarly binds to Hsc/Hsp70. Following transfection of HEK293T cells with either Flag empty vector or Flag-Arc, the whole cell lysates were immunoprecipitated using an anti-Flag antibody. Western analysis using anti-Hsc/Hsp70, anti-Hsp40, and anti-Hsp27 antibodies was used to examine the immunological complex. * was an antibody’s light chain. (F, G) CHIP induces Arc/Arg3.1 degradation by interacting with Hsp70. HEK293T cells overexpressing Flag-Arc, GFP-Hsp70, and His-ubiquitin were co-transfected with HA-CHIP WT or mutants. Cells were analyzed by Western blot with anti-Flag, anti-HA, and anti-GFP antibodies. Representative Western results were selected from triplicated experiments. Quantified results of triplicated Western images are shown in (G). Data are presented as the mean ± S.D. of triplicated experiments (t-test; # < 0.01, **P < 0.001, *** < 0.0001). (H) Arc/Arg3.1 proteins are stabilized by CHIP knockdown. After being depleted of either control or CHIP, MEF cells were exposed to heat shock at 45°C for 15 min and then allowed to recover at 37°C for 9 h.
    Anti Hsp60, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti hsp60/product/Cell Signaling Technology Inc
    Average 97 stars, based on 1 article reviews
    anti hsp60 - by Bioz Stars, 2026-02
    97/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) HSF1-/- cells exhibit reduced expression of heat shock inducible Hsps. WT and HSF1-/- MEF cells were exposed to heat shock at 45 °C for 15 min and allowed to recover at 37 °C for the indicated times. Hsp90, Grp78, Hsc/Hsp70, Hsp60, Hsp40, and Hsp27 were detected by Western blot analysis with their specific antibodies. (B, C) Hsp70 participates in UPS-dependent Arc/Arg3.1 degradation. HSF1-/- MEF cells overexpressing Flag-Hsp70 were exposed to heat shock at 45 °C for 15 min and allowed to recover at 37 °C for the indicated times. Flag-Hsp70 and Arc/Arg3.1 were detected by Western blot analysis using their specific antibodies. Representative Western results were selected from triplicated experiments. Quantified results of triplicated Western images are shown in (C). (D) Hsp70 interacts with Arc/Arg3.1. HEK293T cells were transfected with GFP-Hsp70 and Flag empty vector or Flag-Arc, and the cell lysates were immunoprecipitated using an anti-Flag antibody. The immune complex was analyzed by Western analysis using anti-GFP and anti-Flag antibodies. (E) Arc/Arg3.1 primarly binds to Hsc/Hsp70. Following transfection of HEK293T cells with either Flag empty vector or Flag-Arc, the whole cell lysates were immunoprecipitated using an anti-Flag antibody. Western analysis using anti-Hsc/Hsp70, anti-Hsp40, and anti-Hsp27 antibodies was used to examine the immunological complex. * was an antibody’s light chain. (F, G) CHIP induces Arc/Arg3.1 degradation by interacting with Hsp70. HEK293T cells overexpressing Flag-Arc, GFP-Hsp70, and His-ubiquitin were co-transfected with HA-CHIP WT or mutants. Cells were analyzed by Western blot with anti-Flag, anti-HA, and anti-GFP antibodies. Representative Western results were selected from triplicated experiments. Quantified results of triplicated Western images are shown in (G). Data are presented as the mean ± S.D. of triplicated experiments (t-test; # < 0.01, **P < 0.001, *** < 0.0001). (H) Arc/Arg3.1 proteins are stabilized by CHIP knockdown. After being depleted of either control or CHIP, MEF cells were exposed to heat shock at 45°C for 15 min and then allowed to recover at 37°C for 9 h.

    Journal: bioRxiv

    Article Title: Disulfide Crosslinking Induces Rapid Degradation of Arc/Arg3.1 via Hsp70-Mediated Ubiquitin Ligase Pathway

    doi: 10.1101/2025.07.20.665809

    Figure Lengend Snippet: (A) HSF1-/- cells exhibit reduced expression of heat shock inducible Hsps. WT and HSF1-/- MEF cells were exposed to heat shock at 45 °C for 15 min and allowed to recover at 37 °C for the indicated times. Hsp90, Grp78, Hsc/Hsp70, Hsp60, Hsp40, and Hsp27 were detected by Western blot analysis with their specific antibodies. (B, C) Hsp70 participates in UPS-dependent Arc/Arg3.1 degradation. HSF1-/- MEF cells overexpressing Flag-Hsp70 were exposed to heat shock at 45 °C for 15 min and allowed to recover at 37 °C for the indicated times. Flag-Hsp70 and Arc/Arg3.1 were detected by Western blot analysis using their specific antibodies. Representative Western results were selected from triplicated experiments. Quantified results of triplicated Western images are shown in (C). (D) Hsp70 interacts with Arc/Arg3.1. HEK293T cells were transfected with GFP-Hsp70 and Flag empty vector or Flag-Arc, and the cell lysates were immunoprecipitated using an anti-Flag antibody. The immune complex was analyzed by Western analysis using anti-GFP and anti-Flag antibodies. (E) Arc/Arg3.1 primarly binds to Hsc/Hsp70. Following transfection of HEK293T cells with either Flag empty vector or Flag-Arc, the whole cell lysates were immunoprecipitated using an anti-Flag antibody. Western analysis using anti-Hsc/Hsp70, anti-Hsp40, and anti-Hsp27 antibodies was used to examine the immunological complex. * was an antibody’s light chain. (F, G) CHIP induces Arc/Arg3.1 degradation by interacting with Hsp70. HEK293T cells overexpressing Flag-Arc, GFP-Hsp70, and His-ubiquitin were co-transfected with HA-CHIP WT or mutants. Cells were analyzed by Western blot with anti-Flag, anti-HA, and anti-GFP antibodies. Representative Western results were selected from triplicated experiments. Quantified results of triplicated Western images are shown in (G). Data are presented as the mean ± S.D. of triplicated experiments (t-test; # < 0.01, **P < 0.001, *** < 0.0001). (H) Arc/Arg3.1 proteins are stabilized by CHIP knockdown. After being depleted of either control or CHIP, MEF cells were exposed to heat shock at 45°C for 15 min and then allowed to recover at 37°C for 9 h.

    Article Snippet: Mouse monoclonal Arc antibody (E-7) (sc-55475), mouse monoclonal Arc antibody (C-7) (sc-17839), mouse monoclonal anti-β-Actin (C4) (sc-47778), mouse monoclonal HSP27 antibody (sc-13132), and mouse monoclonal α-tubulin antibody (sc-8035) were purchased from Santa Cruz: Rabbit polyclonal GAPDH antibody (LF-PA0018) and mouse monoclonal HA antibody (LF7H5) (LF-MA0048) from Ab Frontier: Mouse monoclonal Hsc/Hsp70 antibody (ADI-SPA-820) and mouse monoclonal HSF1 antibody (ADI-SPA-901) from Enzo: Mouse monoclonal FLAG antibody (F3165) from Sigma Aldrich: Mouse monoclonal GFP antibody (A-11120) from Invitrogen: Streptavidin-HRP (3999), rabbit monoclonal BiP antibody (C50B12) (3177), and rabbit polyclonal Hsp40 antibody (4868) from Cell signaling: Mouse monoclonal Hsp90 antibody (ab13492), rabbit polyclonal Hsp60 antibody (ab13492), and rabbit polyclonal Hsp60 antibody (ab46798) from Abcam: Goat mouse IgG-HRP conjugate antibody (170–5047) and goat rabbit IgG-HRP conjugate antibody (170–6515) from Bio-Rad.

    Techniques: Expressing, Western Blot, Transfection, Plasmid Preparation, Immunoprecipitation, Ubiquitin Proteomics, Knockdown, Control